Zone electrophoresis with starch-gel and cyanogum-gel

Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

Caeruloplasmin as demonstrated by starch gel electrophoresis.

Starch gel electrophoresis has demonstrated that graded concentrations of sodium sulphate do not precipitate the serum globulins in any reasonable degree of purity. The use of this form of electrophoresis has shown that caeruloplasmin is associated with a globulin in the Fa(2) position.This technique can be employed as a screening test for patients suspected of suffering from hepatolenticular d...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

A modified method for protein separation by zone electrophoresis on a starch gel.

In the present paper (1) a method of preparative zone electrophoresis (cf. 2, 3) is described in which a stiff starch gel with only 7 to 8 per cent of solid constituents is used as a supporting medium. Two simple devices will be described, one for the fractionation of 2 ml. of protein solution and the other for processing up to 50 ml. of solution, containing 5 to 10 per cent of protein mixture....

Gel Electrophoresis

Electrophoresis is the migration of charged particles or molecules in an electric field. This occurs when the substances are in aqueous solution. The speed of migration is dependent on the applied electric field strength and the charges of the molecules. Thus, differently charged molecules will form individual zones while they migrate. In order to keep diffusion of the zones to a minimum, elect...